Consistency of the drug-target proteins profile in human tumor tissues and cell lines of colorectal carcinoma based on the human protein database

Joint Event on 3rd World Congress on NATURAL PRODUCTS CHEMISTRY AND RESEARCH & 12th WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary

Yi-wen Zhang, Luo Fang, Zong-fu Pan and Ping Huang

Zhejiang Cancer Hospital, China

Posters & Accepted Abstracts: Am J Ethnomed

DOI: 10.21767/2348-9502-C1-003

Abstract

Molecularly targeted therapy is the main direction of anti-tumor care. Cell line is wildly used in the preclinical researches of molecularly target drug. While, the consistency of drug-target proteins profile between the human tumor tissues and human tumor cell lines is remained uncertain. In our study, we compared the expression level of drug-target proteins in patients’ colorectal carcinoma tissue with Caco-2 cell line from human protein database to clarify the consistency. The protein expression levels of FDA-approved target-proteins involving in both colorectal carcinoma patients and cell lines were scored base on the intension and quantity of immunohistochemical stain from the database of Human Protein Atlas. The protein expression between individual and cell line was compared. Then the consistency profiles of total proteins between individual and cell line were evaluated. Ultimately, proteins with well or poor consistent expression were identified analyzed by Gene ontology and KEGG enrichment. The expression levels of 176 target proteins involving in both Caco-2 cell line and colorectal carcinoma patients (n=104) were obtained and analyzed. Almost 57.4% of proteins in individual patients were consistent to cell line, which was independent from individual characteristics, such as age, gender and tumor location. About 40% and 47% of total protein, included 47 and 36 proteins with entirely consistent and inconsistent profile, were well and poor consistent expression between patients and cell line, respectively. Those inconsistent proteins were enriched in the pathways related to various types of cancer, immune, extracellular matrix receptor interactions and cytoskeleton. There was a significant difference in the target protein expression between Caco-2 cell line and colorectal tissue, the results suggest that the consistency was important to investigate cell as the model in drug preclinical development.

Biography

Zhang Yi-Wen has her expertise in Pharmacogenetics on the in vivo process of chemotherapy drugs and endogenous substances, quantitative pharmacology research based on population pharmacokinetics. She participated in the construction of important new drug discovery platform of high throughput screening and evaluation of drug metabolism in vitro in cellular and molecular level and response to the several study of Phase I clinical trial. Now, she serves as youth committee member of chemotherapy pharmacological professional committee of Chinese pharmacological society and the precision medical branch of provincial translational medicine institute. She has undertaken four research projects, including national natural science foundation for young.

E-mail: [email protected]