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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13
th
Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X
Page 25
Ag-doped PCL nanofibers for tissue engineering
Permyakova Elizaveta
1
, Manakhov Anton
1
, Sheveyko Alexander
1
Polčak Josef
2,3
, Zajíčková Lenka
4
, Kovalskii Andrey
1
, Ignatov Sergey
5
Shtansky Dmitry
1
1
National University of Science and Technology “MISiS”, Russia
2
Institute of Physical Engineering, Brno University of Technology, Czech Republic
3
CEITEC-Central European Institute of Technology, Brno University of Technology, Czech
Republic
4
RG Plasma Technologies, CEITEC – Central European Institute of Technology, Masaryk
University, Czech Republic
5
State Research Center for Applied Microbiology and Biotechnology, Russia
P
oly-ε-caprolactone (PCL) is a biocompatible and
biodegradable polymer that is attracting great interest
as the promising materials for various applications is
in medicine and, in particular, in tissue engineering.
Here, we produced PCL nanofibers by electrospinning
technique that allows one to obtain the nanofiber
structure similar to that of extracellular matrix. The PCL
scaffolds can be used as bone fillers and skin bandages.
To improve bioactivity and to endow the PCL nanofibers
with antibacterial properties, the material was first
coated with multifunctional bioactive nanostructured
films and then implanted with Ag ions. To select Ag ion
energy, SRIM (The Stopping and Range of Ions in Matter)
calculations were carried out. Microstructure and phase
composition of modified fibers were studied by means
of scanning electronmicroscopy and X-ray photoelectron
spectroscopy. The adhesion and proliferation of the
MC3T3-E1 cells cultivated on the surface of TiCaPCON-
coated PCL nanofibers were significantly improved
in comparison with the uncoated nanofibers. The
antimicrobial effect of the Ag-doped samples was
evaluated against clinically isolated Escherichia coli U20
(E. coli), Staphylococcus aureus 839 (S. aureus) bacteria
and different strains of Neurospora crassa (N. crassa)
Wt987, Nit-6 and Nit 20. In all cases surface Ag-doped
nanofibers had strong antibacterial effect, however Ag
ions didn’t release from the scaffold that means they
don’t be accumulated in the liver. Inductively coupled
plasma mass spectrometry (ICP-MS) which was utilized
to determine the amount of Ag ions leached from the
scaffolds indicated less than 5 ppb/cm
2
released Ag ions
for 7 days.
Biography
Permyakova Elizaveta is a PhD student of Material Technol-
ogy in National University of Science and Technolgy “MISiS”.
The main topic of her research is related to the deposition of
bioactive multicomponent thin films, immobilization of bio-
molecules and surface analysis. Her work is absolutely es-
sential for the development of novel bioactive materials used
in regenerative medicine. Permyakova has already published
eleven articles indexed in Scopus and she is first author of four
articles. Permyakova has demonstrated very good expertise in
biochemistry, cell biology and material characterization.
permyakova.elizaveta@gmail.comPermyakova Elizaveta, J Biomedical Sci 2019, Volume 08