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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 27
Photosensitive tetraether lipid-based liposomes for
temoporfin mediated photodynamic therapy to cancer cells
Sajid Ali, Umair Amin, Jens Schäfer, Jarmila Jedelská and Udo Bakowsky
University of Marburg, Germany
Photodynamic therapy (PDT) is a minimally-invasive
therapeutic approach that is being widely used for
the treatment of large number of medical conditions.
The principal of PDT is based on the combination of a
light sensitive molecule (a photosensitizing agent) and
light. After being administered, the photosensitizer
compound can be illuminated by specific wavelength of
light to activate the drug molecule (photosensitization).
After absorption of light energy of particular wavelength,
the photoactivated sensitizer interacts with molecular
oxygen to generate free radicals and singlet oxygen
species. These highly reactive oxygen species (ROS)
then induce cellular apoptosis or necrosis leading to
tumor destruction. These species are very short lived;
therefore, the resultant tissue damage occurs very
close to production site. Temoporfin loaded liposomes
are prepared by thin film hydration and filter extrusion
technique using stable tetraether lipid combinations.
These liposomes were extruded to get uni-dispersed
liposomal population. These processed liposomes
were characterized for size distribution parameters,
encapsulation efficiency and morphological studies
using dynamic light scattering, laser doppler velocimetry,
ultracentrifugation and atomic force microscopy. These
liposomes were further evaluated for in-vitro photo-
cytotoxicity and intracellular localization with CLSM in
SK-OV-3 cell line. The safety profile of these formulations
was also tested using haemocompatibility assay and in-
vitroCAMmodel. All liposomal formulations ranged from
109 nm to 140 nm in size with a PDI less than 0.2 and
surface charge from -6 to +35mV. Photodynamic studies
showed a dose dependent effect with no cytotoxicity in
unirradiated formulations. Intracellular uptake studies
confirmed the temoporfin localization into the nuclear
region. In vivo CAMmodel showed a strong occlusion of
blood vessel while haemocompatibility studies showed
no toxicity to the blood cells. Present study concludes
that stable liposomes containing temoporfin can be
formulated using different lipid combinations. These
formulations are superior to free temoporfin in terms
of safety and efficacy as well as very effective against
different cancer and bacterial strains.
Recent Publications
1. Mahmoud, G., et al., Stabilized tetraether lipids-
based particles guided porphyrins photodynamic
therapy. Drug delivery, 2018. 25(1): p. 1526-1536.
2. Duse, L., et al., Low level LED photodynamic
therapy using curcumin loaded tetraether
liposomes. European Journal of Pharmaceutics and
Biopharmaceutics, 2018. 126: p. 233-241.
3. Plenagl, N., et al., Hypericin Loaded Liposomes for
Anti‐Microbial Photodynamic Therapy of Gram‐
Positive Bacteria. physica status solidi (a), 2018.
215(15): p. 1700837.
Sajid Ali et al., J Biomedical Sci 2019, Volume 08