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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13
th
Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X
Page 21
Characterization of three-dimensional bone constructs
derived from unloaded human fetal osteoblasts exposed to
the random positioning machine and use of prebiotics for
bone heath.
Alamelu Sundaresan
1
, Vivek Mann
2
, Sundar Devakottai
3
, Ria Devakottai
4
,
and
Daniella Grimm
5
1
Texas Southern University, USA
2
University of Incarnate Word School of Medicine, USA
3
Texas A and M University, USA
4
Arhus University, USA
H
uman cells exposed to microgravity form large 3D
tissue constructs mirroring the
in vivo
architecture
(e.g. cartilage, intima constructs, cancer spheroids
and others). In this study, we exposed human fetal
osteoblasts (hFOB 1.19) cells to the Random Positioning
Machine (RPM) for 7 and 14 days with the purpose to
engineer 3D bone constructs. RPM-exposure of hFOB
1.19 cells induces alterations in the cytoskeleton, cell
adhesion, ECM and 3D multicellular spheroid (MCS)
formation. In addition, it also influences the morphologic
appearance of these cells after 7 days as it forces
adherent cells to detach from the surface and assemble
in 3D structures. The RPM-exposed hFOB 1.19 cells
exhibited a differential gene expression of the following
genes: transforming growth factor beta 1 (TGFB1), bone
morphogenetic protein 2 (BMP2), SRY-Box 9 (SOX9),
actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM),
laminin subunit alpha 1 (LAMA1), collagen type 1 alpha
1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin
1(FN1). RPM-exposure also induced significantly
altered release of the cytokines and bone biomarkers
sclerostin (SOST), osteocalcin (OC), osteoprotegerin
(OPG), osteopontin (OPN), interleukin 1 beta (IL-1) and
tumor necrosis factor 1 alpha (TNF-1). After two weeks
of incubation, the spheroids presented a bone-specific
morphology. Of late unloading conditions and use of
prebiotics are known to augment 3D tissue engineering
of immune cells and bone. Preliminary results from the
use of a prebiotic AHCC on lymphocytes and hFOB cells
in unloaded conditions will also be presented.
Recent Publications
1. A. Sundaresan, S.Devakottai, J. E. Reseland: Effects
of load on normal human osteoblast function.
European Cells and Materials,. Volume No 26 -
Supplement 2,pages 32-33 – 2013.
2. Claudia Ulbrich,1 Markus Wehland,2 Jessica
Pietsch,2 Ganna Aleshcheva,2 Petra Wise,3
Jack van Loon,4,5,6 Nils Magnusson,7 Manfred
Infanger,2 Jirka Grosse,8 Christoph Eilles,8
Alamelu Sundaresan,9 and Daniela Grimm10.
Review Article. The Impact of Simulated and Real
Microgravity on Bone Cells and Mesenchymal
Stem Cells. BioMed Research International.Volume
Alamelu Sundaresan et al., J Biomedical Sci 2019, Volume 08