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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 24
Induction of hepatic regeneration in an experimental model
using hepatocyte differentiated MSCs
Taghrid Gaafar
Cairo University, Egypt
Background and Objectives: Scaffolds are three-
dimensional (3D) matrices that provide support for
cells to attach, proliferate, and differentiate, facilitating
extracellular matrix formation. The study aimed to
examine the differentiation potential of Mesenchymal
stem cells (MSCs) into hepatocytes in 2D and 3D culture
systems to improve their in vitro differentiation, and test
their functionality in vivo.
Methods: MSCs were generated from umbilical cord
blood. Hepatogenic differentiation was induced on
2D and 3D cultures and characterized by morphology,
scanning electron microscopy, immunocytochemistry
and Gene expression. Albumin and α-1 antitrypsin (AAT)
in culture supernatants were measured. Differentiated
Cells were administered IV into amurinemodel of carbon
tetra (CCL4) induced liver cirrhosis which were divided
into 3 groups, a) Pathological control group, b) and c)
Groups treated with hepatogenic differentiated MSCs
cultured on 2D and 3D culture system respectively. After
12 weeks of injection, liver pathology was examined.
Results: The hepatogenic differentiated MSCs stained
positively for albumin, alpha fetoprotein (AFP), Heppar1,
cytokeratin7, 18, and OV6 with more mature cells,
hexagonal in shape with central nuclei forming large
sheets in groups in 3D culture system. AAT secretion and
Indocyanine green uptake were significantly increased
in 3D system. In experimental model, MSC-3D treated
group exhibited maximal restoration of liver architecture
with absent septal fibrosis and marked improvement of
ALT, AST.
Conclusions: Both 3D and 2D culture systemare effective
in functional hepatogenic differentiation from MSCs. In
vivo hepatogenic differentiation is more effective on 3D
scaffold, with better functional recovery.
Biography
Roberta Di Pietro got the degree in Medicine cum Laude in
1985 and the Specialization in Sports Medicine cum Laude in
1988, University of Chieti, Italy. She worked as a Visiting Scien-
tist at the Biochemistry Department, AFRC, Cambridge, UK; at
the Pathology Department, USUHS, Bethesda, USA, and at the
Institute of Human Virology, University of Maryland, Baltimore,
USA. She got the position of Full Professor of Histology and
Embryology at the University of Chieti since 2005. She joined
the Editorial Board of Current Pharmaceutical Design as an
Executive Guest Editor and, recently, the Editorial Academy of
the International Journal of Oncology as an Honorary Member.
She was recognized as a Registered Referee for Archives of
Ophthalmological Reviews and Reproductive Biology and En-
docrinology. She is now author of 200 scientific publications
plus international e-book chapters, editorials, Italian textbooks
and 1 Italian patent.
Taghrid Gaafar, J Biomedical Sci 2019, Volume 08