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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988 Plant Science | Tissue Engineering | Parasitology 2018
Biography
Zhong yanting, is pursuing his PhD from College of resources
and environmental sciences, China Agricultural University,
Beijing, China. His research broadly focuses on Molecular
Biology of Plant Nutrition, and currently working on Phosphorus
.
In our study, overexpression of ZmCCD8b, a paralogous gene of ZmCCD8a, in
Arabidopsis mutant max4 failed to rescue the mutant phenotype. However,
we found ZmCCD8b expression is significantly higher in silk, while relative
higher in ear and root under low phosphorus (LP) condition reciprocal to
control (sufficient nutrients) reaching the highest level on the tenth day.
The expression was stronger in pericycle of the meristematic zone and the
elongation zone as revealed by In situ hybridization. Protoplast amplification
showed that this gene was localized to the plastid. We used virus induced gene
silencing (VIGS) technology to silence ZmCCD8b and the results indicated that
in contrast to GFP control, the carotenoid contents significantly reduced in
leaves of Zmccd8b lines. In contrast to increased phosphorus concentration
in root whereas, decreased phosphorus concentration in shoot, root biomass
of Zmccd8b silenced lines non-significant. While shoot dry weight decreased
significantly as compared to GFP control, contributing to an increased root
to shoot ratio of Zmccd8b silenced lines. Furthermore, ZmCCD8b may affect
the expression of PHO2 and PHO1 through PHRs. Additionally, ZmPht1;1 and
ZmPht1;6 were down-regulated whereas, ZmPht1;3 and ZmPht1;13 were up-
regulated in Zmccd8b silenced lines under LP conditions. Yeast one-hybrid
and EMSA experiments verified that ZmPHR1s can regulate ZmCCD8b through
P1BS element. Transcriptome analysis indicated increased expression of
genes related to stress and signal transduction in ZmCCD8b heterologous
overexpression lines.
Zhong Yanting, Xiaoying Pan, Ruifeng Wang, Faisal Nadeem,
Jianyu Zhao, Yanjun Xu, Xuexian Li
China Agricultural University, China
Zhong Yanting et al., Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006
ZmCCD8b encodes a carotenoid cleavage
dioxygenase regulating the plant response to
phosphorus limitation