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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988
Plant Science | Tissue Engineering | Parasitology 2018
Biography
Ruifeng Wang is pursuing his PhD (third year) fromDepartment
of Plant Nutrition, China Agricultural University. His research
works are focused on the molecular basis of phosphate
deficiency to the developing maize ear and characteristics
of AUX/IAA involved in the root development under nitrogen
limitation. Recently, he is doing analysis of fine mapping genes
related to maize root architecture traits with a RIL population.
He has published three papers in reputed journals as a co-
author of which one is focused on maize ear development
under nitrogen deficiency and the other two on the root
morphological modification of foxtail millet under N and Pi
deficiency respectively. His interest is on plant genomics and
molecular breeding.
wangruifeng@cau.edu.cnM
aize grain yield is depending on ear development and kernel number
which is associated with the crucial silking stage. However, low P-caused
consequences in developing maize ear remains elusive. Morphological
alteration in the Pi deficient ear showed less kernel number but no less
longitudinal growth. A cut down of soluble proteins, free amino acids and
variation in hormone profiling, especially a significant drop in IAA accumulation
were examined in low P feed ear as well. Transcriptional analysis uncovered
847 genes differentially expressed, with 423 genes induced and 424 genes
repressed, whichwerecharacterizedof genes related tohormones, transcription
factors and the dominating repression of genes in transport category. To further
investigate if those genes are directly bound to P deficiency, 313 out of 847
genes were found harbouring PHR1 Binding Sequence (P1BS) and featured in
the pathways of Nmetabolism, lipid metabolism and photosynthesis indicating
the wide spectrum consequences caused by Pi deficiency and the direct
cross-talk between phosphorus and nitrogen signalling pathway. Furthermore,
with yeast one-hybrid assay and EMSA, we detected the interaction between
ZmPHR1 and P1BS at the promoter region of two amino acid transporters,
ZmAAP2 and ZmLHT1,which function in transport of several key amino acids,
and ZmPHR1 represses the expression of ZmAAP2 and ZmLHT1, consolidating
the potential interaction between N and P. Taken together, these results suggest
that the kernel abortion caused by Pi deficiency was associated with hormones
variation and may intensively connected with the overall down regulation of
genes in transport and especially the alteration of N metabolism.
Kernel abortion is coupled with cis-regulation of
ZmAAP2 and ZmLHT1 by ZmPHRs in the phosphate
limited maize ear
Ruifeng Wang and Xuexian (Steve) Li
China Agricultural University, China
Ruifeng Wang et al., Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006