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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988 Plant Science | Tissue Engineering | Parasitology 2018
Schistosomiasis is a parasitic disease that causes severe pathology,
mortality and economic loss worldwide. It is especially prevalent in Africa.
These parasites require intermediate host (snails) in their life cycles. This
pathogen continues to extend to new geographical regions despite significant
advances in control. To fully understand the dynamics of schistosomiasis
transmission, integration of the ecological factors that affect both humans
and freshwater snails is needed. New diagnostic methods that target extra-
organismal environmental DNA (eDNA) can offer better identification of
human-infecting Schistosoma in epidemiological studies. These methods
could also provide more sensitive detection in low population densities of
the target organisms. Therefore, this study aimed to design and test real-
time qPCR probes and primers for Schistosoma mansoni, S. haematobium
and S. japonicum by amplifying species-specific amplification. The developed
primers were tested on microcosm eDNA samp`les with confirmed S. mansoni
presence in the cultures of gastropods. These developed primers and probes
successfully identified the presence of Schistosoma within eDNA from the
natural environment (Tanzania), thereby indicating that eDNA monitoring is a
valid method for the detection of Schistosoma in freshwater bodies.
Biography
Hind A Alzaylaee has completed her Masters’ Degree from Taif
University, Saudi Arabia and she is currently doing her PhD at
University of Bristol. She is a Lecturer of Parasitology, Princess
Nourah Bint Abdulrahman University, Saudi Arabia.
Can Schistosoma be detected by environmental DNA?
Hind A Alzaylaee1, Martin J Genner1 and Eric R Morgan2
1University of Bristol, UK
2Queen’s University Belfast, UK
Hind A Alzaylaee et al., Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006