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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988 Plant Science | Tissue Engineering | Parasitology 2018
Biography
Huilan Luo has completed her Undergraduate degree from
Shihezi University and Masters’ degree from China Agriculture
University. Currently, she is a PhD student in Department
of Plant Nutrition, College of Resources and Environmental
Sciences, China Agricultural University, China.
Maize (Zea mays L.) plays a significant role in global food security and
energy consumption. Nitrogen (N), an important macronutrient, exists
in soils heterogeneously both as inorganic and organic form. Amino acids,
a crucial organic nitrogen source for plants, are not only the structural
components of biological proteins but also have biological functions to
regulate hormone synthesis, nitrogen transport, resistance to external abiotic
stress, and root morphology. Glutamine is an important intermediate in plant
nitrogen metabolism. The nitrogen absorbed by plants from the soil is first
converted to glutamine, and then to other biomolecules vital for various
biological activities of plants. In addition, glutamine as a metabolite of NO is
a negative signal for plants to absorb nitrate and induce nitrogen metabolism.
Therefore, understanding the molecular and physiological mechanisms of
amino acid absorption and transport in maize, using emerging biotechnological
and analytical methods, is of great significance. There are large differences
in the genomes of maize, which provide a good opportunity to study them
using genome-wide association analysis (GWAS). With the development of
high-throughput sequencing technology and the application of correlation
analysis of plants, genetic basis of amino acid absorption in maize can be
further analysed. Use of GWAS combined with genomics and metabolomics
and other big data analysis methods for the analysis of amino acid absorption
and metabolism in maize becomes worth studying. This study used 510 maize
inbred lines under control and glutamine treatment. We found phenotypic
indicators related trait of maize varied broadly. There was 2.23-8.96 fold change
and 7.76-18.48 fold change SPAD value, shoot dry weight, root dry weight, total
dry weight, root length and root to shoot ratio as a whole under control and
glutamine treatment, respectively. A set of 27229 high quality SNPs were used
to perform GWAS on the diverse panel of 510 maize inbred lines. 6 genetic loci
were identified in control whereas 5 genetic loci were identified in glutamine
treatment at p≤3.35×10-6 significant level.
Whole genome wide association analysis of maize
(Zea mays L.)in response to glutamine
Huilan Luo, Faisal Nadeem and Xuexian Li
College of resources and environmental sciences-China Agricultural University, China
Huilan Luo et al., Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006