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Volume 4, Issue 2 American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3rd World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12th WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
The inhibitory effect of licorice roots on IFN-γ-mediatedmicroglia stimulation and the reactive mouse
splenic T cell responses
Eun-Ju Yang and Kyung-Sik Song
Kyungpook National University, Republic of Korea
Multiple Sclerosis (MS) is an autoimmune neurodegenerative disorder in the Central Nervous System (CNS), and the
immune responses induced by auto reactive T cells are the hallmark in the development of MS. The autoreactive T
cells overproduce the pro-inflammatory cytokines, such as interferon-γ (IFN-γ) leading to the microglia stimulation via
the CXCL10 (IP-10) chemokine production. The licorice roots (Glycyrrhizae Radix, GR) have been investigated for various
biological activities, however, the suppressive effect of GR on IFN-γ-stimulated microglia BV2 and the reactive mouse splenic
T cell responses have not been reported yet. In this study, the GR ethanolic extract and its partitions were treated to BV2 cells
and mouse splenic T cells to determine the anti-inflammatory effects. Ten ng/mL IFN-γ significantly enhanced the nitric oxide
and Tumor Necrosis Factor (TNF)-α productions in BV2 cells, however, it was effectively inhibited by not only GR extract
but also the Dichloromethane (MC) and Ethyl Acetate (EA) fractions of GR. In addition, MC and EA fractions decreased
the IP-10 production. On the other hand, when the mouse splenocytes were stimulated with anti-CD3 and anti-CD28 for 3
days, GR extract reduced the populations of IL-17A+IFN-γ+ and IL-17A-IFN-γ+ in CD4+ and CD8+ T cells. The IL-17A,
IFN-γ, and IL-6 productions were decreased, as well. By MC and EA fractions, IL-17A+IFN-γ+CD4+, IL-17A-IFN-γ+CD4+,
and IL-17A-IFN-γ+CD8+ T cell populations were effectively decreased, and the productions of IL-17A and IFN-γ were also
suppressed. Especially, MC fraction significantly reduced the populations of Tbet+IFN-γ-, Tbet+IFN-γ+, and Tbet-IFN-γ+
in CD8+ cells, when the CD3+ T cells were polarized into TH1 and TC1 cells. The elevated IL-17A, IFN-γ, IL-6, TNF-α, and
CXCL10 productions were also decreased. These data suggest that GR and its fractions could be useful for anti-inflammatory
effect on microglia and T cells in the CNS autoimmune condition, especially MS.
Biography
Eun-Ju Yang is a professor at Kyungpook National University, Republic of Korea. He has his expertise in Natural Products and Medicinal Chemistry.
Eun-Ju Yang et al., American Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003