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Volume 4, Issue 2 American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3rd World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12th WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Activity of novel semisynthesized sesquiterpene lactones towards NF-B in a her2 breast cancer cell line
Wendy Soria1,3, Rodrigo Villagomez1, Maribel Lozano1,2, Giovanna Almanza2, Olov Sterner1, and Stina Oredsson1
1Lund University, Sweden
2Universidad Mayor de San Andrés, Bolivia
3Universidad Mayor de San Andres, Bolivia
The NF-B signalling pathway is constitutively activated in Cancer Stem Cells (CSCs) inducing the expression of genes
that regulate proliferation, invasion, and metastasis. Plant-derived Sesquiterpene Lactones (SLs) have been suggested to
inhibit this transcription factor. SLs are found in plants from the Asteraceae family and one species in this family, Ambrosia
arborescens, contains a high concentration of damsin. We have previously shown that treatment with damsin reduces the
CSC population of the HER2 breast cancer cell line JIMT-1. Damsin was used as a starting material for the synthesis of the SL
analogue DCS3 followed by the synthesis of four DCS3 SL analogues. The toxicity of DCS3 and the analogues was investigated
in the normal-like human breast epithelial cell line MCF-10A and in the JIMT-1 breast cancer cell line. Inhibitory concentration
50 (IC50) values were obtained fromMTT-based dose response curves. Immunofluorescence microscopy was used to evaluate
if the compounds inhibited TNF-induced translocation of NF-B to the cell nucleus. All compounds were more toxic to JIMT-
1 cells than to MCF-10A cells as shown by lower IC50 values in the former cells. DCS3 was more toxic than damsin and the
DCS3 SL analogues were more toxic than DCS3. At IC50, all compounds inhibited TNF-induced translocation of NF-B to the
cell nucleus. The compounds reduce the CSC sub-population of JIMT-1 cells. Our results suggest that these compounds should
be further investigated to find efficient CSC inhibiting compounds that may be used in the clinic.
Biography
Wendy Soria is a Doctoral student of Functional zoology at Lund University, Sweden. Her research focus in Anti-cancer stem cell activity of a sesquiterpene lactone
isolated from Ambrosia arborescens and of a synthetic derivative.
Wendy Soria et al., American Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003