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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Breeding new
Cordyceps militaris
strain using mating type molecular markers
Miae Lee
and
Byungjoo Lee
CARES, South Korea
An entomopathogenic
Cordyceps militaris
is being studied and cultivated as a medicinal mushroom having many valuable biological
and pharmaceutical activities substituting
C. sinensis
which has been traditionally used as a Chinese herb medicine.
C. militaris
can
be cultivated artificially. As a bipolar heterothallic fungus
C. militaris
has two strains of compatible mating types, MAT1 and MAT2
which are determined by the single mating type locus MAT1 consisting of two dissimilar alleles called idiomorphs MAT1-1 and
MAT1-2 of MAT1 and MAT2, respectively. They can be differentiated by crossing, fruiting body formation ability and the production
of perithecia. Such process is very laborious and time consuming to carry out but molecular markers of these mating types reduces
the amount of effort required for the crossing process. In this study, two opposite mating types were assayed using two sets of primers
specific for
C. militaries
, which were amplified a 191-bp fragment for MAT1-1 and 233-bp fragment for MAT1-1. After crossing of
two compatible mating types F1 hybrids resulted in well-developed perithecial fruiting bodies and their crossings were confirmed
by the multiplex PCR assays for the rapid and specific detection of both MAT1-1 and MAT1-2. In the breeding process of new
C.
militaris
mushroom, single ascospores were isolated and examined their mating types, mycelial growth, mycelial density, and selected
isolates were crossed and hybrids were produced showing high quality fruiting bodies on artificial media. The stromata of new strain
‘Chungnam 12’ were club-shaped and bright orange-red. Its height was 6.7 cm and the cordycepin content was 0.33% on average.
The new strain showed 11% higher yield than ‘Yedang 3’ with producing firmer fruit bodies. The optimum temperature for mycelial
growth was 22~25
0
C and the optimum temperature for stroma development was 18~22
0
C. Fruiting bodies were begun to produce
43 days later after inoculation. This new cultivar may serve as a valuable one for artificial cultivation and industrial-scale production
of
C. militaris
.
Biography
Miae Lee is an Agricultural Researcher in Crop Research Division, Chuncheongnam-Do Agricultural Research and Extension Services in South Korea for about 10
years and has her expertise in breeding new mushroom cultivars. She is also doing her best in developing various cultivation methods for better quality mushroom
production. Recently, she is involved in environmental friendly disease and pest management, particularly against mushroom flies. She investigates the occurrence
and development of sciarid flies and the use of plant extracts such as
Ginko biloba
fruits to control mushroom flies in button mushroom cultivation
bioran@korea.krMiae Lee et al., Med Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003