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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Anike N F et al., Med Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003
Chemical stressors increase polysaccharide secretion in
Lentinus squarrosulus
Anike F N
1
, Isikhuemhen O S
1
, Obinyan B
2
and
Okhuoya J A
2
1
North Carolina A&T State University, USA
2
University of Benin, Nigeria
Introduction:
Lentinus squarrosulus
is a seasonal mushroomconsumed bymillions of people. Beyond nutritive factors,
L. squarrosulus
elicit medicinal benefits through its polysaccharide content. While attempts at domestication and mass production of
L. squarrosulus
(on going) is one way to increase polysaccharide output, mechanisms that increase polysaccharide secretion
in vitro
is an attractive
alternative both for process optimization and product recovery. The study evaluated the effectiveness of chemical stressors such as
acidity and sodium chloride in increasing polysaccharide secretion in
L. squarrosulus
.
Methodology:
L. squarrosulus
was cultured in submerged fermentation under different concentrations of sodium chloride (0.5, 1,
and 2 g/l) and pH levels (3, 4, 5, 6, 7, 8, and 9) including their controls. The basal medium was composed of soluble starch (10 g/L),
Dextrose (5 g/L) and yeast extract (2 g/L). Five (5) replicates of each medium were inoculated with 5 mm agar blocks from 3-day old
L. squarrosulus
culture and incubated at 28˚C for 14 days. Mycelia were harvested by filtration and polysaccharide precipitated from
supernatant in 3 volume ethanol overnight, centrifuged, dried and weighed.
Result:
The two chemical stressors increased secretion of polysaccharides in
L. squarrosulus
tremendously compared to non-stressed
cultures. Acidic pH was more favorable to polysaccharide secretion than alkaline pH. Highest polysaccharide (0.026g) was detected
in pH 3 and least (0.01g) in pH 6-9. Low sodium chloride concentration (0.5 g/L) resulted in more polysaccharide secretion while
higher salt concentrations (2 and 3 g/L) inhibited growth completely. Acidic medium was more effective in inducing polysaccharide
than low salt medium (0.26 g vs 0.2 g).
Conclusion:
Chemical stress using sodium chloride and acidic pH induced higher polysaccharide secretion in
L. squarrosulus
. The
method could be optimized and adapted for commercial production of
L. squarrosulus
polysaccharide.
Biography
Anike F N is an experienced Researcher and Educator with expertise in mushroom and fungal biotechnology. She conducts research and trains students in this field of study,
authored and co-authored many peer reviewed journals and publications.
fnanike@ncat.edu