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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Evidence that virulent
Cochliobolus lunatus
colonizes potato by down-regulating proteome at late stages
of infection
Bengyella Louis, Pranab Roy
and
Naser Aliye Feto
Vaal University of Technology, South Africa
I
t is confirmed that
Cochliobolus lunatus
adopt different but highly successful strategies on potato cultivars to incite brown-to-black
leaf spot disease.
C. lunatus
abundantly produces four-celled conidia at high temperatures (>30°C) and under suitable conditions;
the fungus colonizes potato (
Solanum tuberosum L
.) cultivars by adopting different invasion strategies at the microscopic level. Long-
lasting defense during infection requires an upsurge in proteome changes particularly pathogenesis related proteins (PrPs) chiefly
under the control of nonexpresser of pathogenesis related proteins. In order to gain molecular insights, we profiled the changes in
proteome and potato nonexpresser of pathogenesis related proteins (
StNPR1
) during the infection process. It is found that
C. lunatus
significantly (P<0.05) suppressed the host functional proteome by 96 hours after infection (hai), principally, affecting the expression
of ribulose bisphosphate carboxylase enzyme, plastidic aldolase enzyme, alcohol dehydrogenase 2 and photosystem II protein prior
to the formation of brown-to-black leaf spot disease. Strongest host–response was observed at 24 hai hallmarked by 307 differentially
expressed peptide spots concurring with the active phase of production of penetrating hyphae. Additionally,
C. lunatus
differentially
down-regulate
StNPR1
transcript by 8.19 fold by 24 hai. This study is the first to elucidate that
C. lunatus
transiently down-regulate
the expression of
StNPR1
at the onset of infection, and as a whole, infection negatively affects the expression of proteome components
involve in photosynthesis, carbon fixation and light assimilation. This study contributes towards better understanding of the
mechanism underlining the invasion strategies of
C. lunatus
.
bengyellalouis@gmail.comMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003