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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Survey bioremediation of chlorophenol by yeast fungi isolated from industrial wastewaters and oil
Atena Alirezaei
1
, Mansour Bayat
1
, Mahmood Alimohmmadi
2
and
Seyedjamal Hashemi
2
1
Islamic Azad University, Iran
2
Tehran University of Medical Science, Iran
Introduction:
The potential of various organisms to metabolize organic compounds has been recognized as potentially effective
means of disposing of hazardous wastes. Among these compounds, phenolic compounds are toxic pollutants which belong to
chlorophenols known as xenobiotic chemicals. They are extensively used as herbicides, insecticides, fungicides, wood preservatives,
resins and lubricants. 4-Chlorophenol (4-CP) is one of the chlorophenols with a high solubility in water, so it is most detected in
wastewater and can accumulate in their bodies biologically.
Methods:
In present study 14 Strains of bacteria and 5 Strains of Yeast and mold phenol degradation was purified from Shahid
Tondgooyan petrochemical wastewater treatment unit was first carried out within about 60 days. Then, capability of the isolated
microorganisms in biodegradation of 100 ppm 4-chlorophenol in presence of 2 and 5 g/l glucose as a growth substrate was examined.
Two microorganisms, selected as superior species. The strains were designated TY1 and TY2 and identification was performed by
sequencing of 18S rRNA and confirmed by morphological and biochemical characterization. Various physicochemical parameters
are optimized for the maximum biodegradation of 4-CP, i.e. pH, temperature, initial concentration of phenol (100, 200 and 300 ppm)
and carbon sources such as glucose (2, 5 g/l) concentrations. The phenol degradation was determined by the spectrophotometric
method 4-amino antipyrine.
Results:
The results showed that 100% removal of 100 ppm by mixed culture of TY1 TY2:50/50 in presence of 2 gr/l glucose within
15 hours. Percentage of pure cultures in mixed culture had no significant effect on 4-CP removal efficiency however; initial glucose
with lower concentration had higher 4-CP removal efficiency. Furthermore, the results of the sequencing showed that the isolates
with the genus
Trichosporon
.
Conclusion:
The significance and impact of the study is the utilization of native yeast strains isolated from the waste water itself
having potential for environmental bioremediation in petroleum refinery and petrochemical industries.
Atena.Alirezaei@gmail.comMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003