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Page 79
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
7
t h
E u r o s c i c o n C o n f e r e n c e o n
Clinical Pathology
and Epidemiology
F e b r u a r y 2 7 - 2 8 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
Clinical Pathology & Epidemiology 2019
Background:
Acinetobacter baumannii
is an opportunistic pathogen causing various nosocomial infections. The aim of this study
was to characterize the molecular support of carbapenem-resistant
A baumannii
clinical isolates recovered from two Libyan
hospitals.
Methods:
Bacterial isolates were identified and antibiotic susceptibility test was performed using automated system.
Carbapenem resistance determinants were studied phenotypically using three different techniques: metallo-β-lactamase (MBL)
E-test; chromogenic culture media and modified Hodge test (MHT). Polymerase chain reaction (PCR) amplification was used to
determine the presence of metallo-β-lactamase blaNDM-1, blaO
XA23
, blaO
XA48
and bla
OXA51
genes among isolates.
Results:
A total of 108
A. baumannii
isolates were characterized, overall the resistance prevalence was extremely high for
aminoglycosides, fluoroquinolones, cepahosporens and carbapenemes (93.2-100%), all isolates were susceptible to colistin.
In addition, 97.5% of isolates were identified as multidrug resistance (MDR). Varying degree of phenotypic detection of
carbapenemes was determined; highest levels of carbapenemes were detected using chromogenic media (75.5%) compared with
MBL E-test (45.5%) and MHT (71.4%). The carbapeneme resistance-encoding genes detected were blaNDM1 (70.6%),
blaO
XA23
(84%),
bla
OXA48 (46.2%) and
bla
OXA51 (73.1%); the highest carbapeneme genes were demonstrated in Burn and Plastic Surgery
Hospital (73.7%). The co-occurrence of blaNDM1,
bla
OXA23 and blaOXA48 genes were demonstrated in (30/119; 25.2%) showing
dissemination of carbapenemes resistance MDR
A. baumannii
in hospitals. MLST analysis for
A. baumannii
isolates revealed also
the presence of multiple clones in our study. The clones belonging to ST1 and ST2 were the most frequent
Conclusion:
This study shows that the high prevalence of NDM-1 and OXA-23 contribute to antibiotic resistance in Libyan
hospitals and represents the high incidence of carbapenemases in an autochthonous MDR
A. baumannii
isolated from patients in
Libya, indicating that there is a longstanding infection control problem in these hospitals.
zorgania@yahoo.comHigh prevalence of metallo-
β
-lactamase
carbapenemase-producing
Acinetobacter
baumannii
in Tripoli, Libya: dominance of OXA-23
and NDM-1
Abdulaziz Zorgani, Nasim Abukhatwa, Nada Elgrew, Balsem
Bezan, Najib Sufya, Abdallah Bashien and Omar Elahmer
University of Tripoli, Libya
J Infec Dis Treat 2019, Volume: 5
DOI: 10.21767/2472-1093-C1-009