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Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
7
t h
E u r o s c i c o n C o n f e r e n c e o n
Clinical Pathology
and Epidemiology
F e b r u a r y 2 7 - 2 8 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
Clinical Pathology & Epidemiology 2019
T
uberculosis (TB) is an important public health.
Mycobacterium tuberculosis
, the etiological agent of tuberculosis is responsible
for maximum mortality by infection diseases globally. Increasing the transmission of MDR and XDR strains of
Mycobacterium
tuberculosis
has created a challenge for effective treatment and disease control. MDR and XDR strains of
Mycobacterium
tuberculosis
evaluate and diagnose different types of mutations in the katG, rpoB, rrs and gyrA genes. Drug susceptibility testing
for Isoniazid, Rifampin, Kanamycin, ethambutol and Streptomycin was performed using the agar proportion method. The regions
of katG, rpoB, rrs and gyrA genes in the MDR and XDR strains are sequenced by PCR and sequencing results at NCBI, BLAST.
There is a relationship between the presence of resistance phenotypes and genetic mutations. Common mutations are found in
the studies. The sequencing of specific regions of target genes is useful for detecting MDR and XDR strains and is also valuable
for the design of novel diagnostic techniques.
elahehajimiri@yahoo.comMolecular detection of XDR and MDR
Mycobacterium tuberculosis
Elahe Sadat Hajimiri
1,2
and Nayereh Ebrahimzade
2
1
Islamic Azad University, Iran
2
Pasteur Institute of Iran, Iran
J Infec Dis Treat 2019, Volume: 5
DOI: 10.21767/2472-1093-C1-009