Page 67
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
7
t h
E u r o s c i c o n C o n f e r e n c e o n
Clinical Pathology
and Epidemiology
F e b r u a r y 2 7 - 2 8 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
Clinical Pathology & Epidemiology 2019
Aim:
The aim of this study was to compare the diagnositc values of two different molecular based tests (GenoType® HelicoDR ve Seeplex®
H. pylori
-ClaR- ACE Detection) in detection presence of the
H. pylori
from gastric biopsy specimens. In addition to this, we also aimed to
determine resistance ratios of
H. pylori
strains against to clarytromycine and quinolone isolated from gastric biopsy material cultures by
using both the genotypic (GenoType® HelicoDR, Seeplex ®
H. pylori
-ClaR-ACE Detection) and phenotypic (gradient strip, E-test) methods.
Material & Methods:
A total of 266 patients who admitted to Konya Education and Research Hospital, Department of Gastroenterology with
dyspeptic complaints, betweenJan’ 2011-Jun’ 2013, were included in the study.Microbiological andhistopathological examinationsof biopsy
specimens taken from antrum and corpus regions were performed. The presence of
H. pylori
in all the biopsy samples was investigated by
five differnt dignostic methods together: culture (C) (Portagerm pylori-PORT PYL, Pylori agar-PYL, GENbox microaer, bioMerieux, France),
histology (H) (Giemsa, Hematoxylin and Eosin staining), rapid urease test (RUT) (CLOtest, Cimberly-Clark, USA) and two different molecular
tests; GenoType® HelicoDR, Hain, Germany, based on DNA strip assay, and Seeplex ®
H. pylori
-ClaR- ACE Detection, Seegene, South
Korea, based on multiplex PCR. Antimicrobial resistance of
H. pylori
isolates against clarithromycin and levofloxacin was determined by
GenoType® HelicoDR, Seeplex ®
H. pylori
-ClaR- ACE Detection, and gradient strip (E-test, bioMerieux, France) methods. Culture positivity
alone or positivities of the both histology and RUT together was accepted as gold standard for
H. pylori
positivity. Sensitivity and specificity
rates of two molecular methods used in the study, were calculated by taking the two gold standards previously mentioned.
Results:
A total of 266 patients between 16-83 years old, out of which 144 (54.1%) were female, 122 (45.9%) were male were included
in the study. 144 patients were found as culture positive and 157 were H and RUT were positive together. 179 patients were found as
positive with GenoType® HelicoDR and Seeplex ®
H. pylori
-ClaR-ACE Detection together. Sensitivity and specificity rates of studied five
different methods were found as fallows: C were 80.9% and 84.4% , H + RUT were 88.2% and 75.4%, GenoType® HelicoDR were 100 %
ve 71.3 %, and Seeplex ®
H. pylori
-ClaR- ACE Detection were, 100% and 71.3%. A strong correlation was found between C and H+RUT, C
and GenoType® HelicoDR, and C and Seeplex ®
H. pylori
-ClaR-ACE Detection (r=0.644 and p=0.000, r=0.757 and p=0.000, r=0.757 and
p=0.000, respectiveley). Of all the isolated 144,
H. pylori
strains, 24 (16.6%) were detected as resistant to clarithromycin and 18 (12.5%)
were levofloxacin. Genotypic clarithromycin resistance were detected only in 15 cases with GenoType® HelicoDR and 6 cases with Seeplex
®
H. pylori
-ClaR- ACE Detection.
Conclusion:
In this study it was cocncluded that GenoType® HelicoDR and Seeplex ®
H. pylori
-ClaR-ACE. Detection was found as the most
sensitive diagnostic methods when compared to all the investigated other ones (C, H, and RUT).
recepkesli@gmail.comA diagnositc accuracy study: comparison of two
different molecular based tests (GenoType Heli-
coDR and Seeplex ClaR-
H. pylori
ACE detection), in
the diagnosis of
Helicobacter pylori
infections
Recep Kesli
1
, Huseyin Bilgin
2
, Yasar Unlu
3
and Gokhan Gungor
3
1
Selcuk Universit, Turkey
2
Uludag University, Turkey
3
Saglık Bilimleri University, Turkey
J Infec Dis Treat 2019, Volume: 5
DOI: 10.21767/2472-1093-C1-009