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Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
7
t h
E u r o s c i c o n C o n f e r e n c e o n
Clinical Pathology
and Epidemiology
F e b r u a r y 2 7 - 2 8 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
Clinical Pathology & Epidemiology 2019
S
warming motility plays important roles in the pathogenicity of
Pseudomonas aeruginosa
, a frequent opportunistic human
pathogen. Bacterial swarming is coordinated by quorum sensing, a cell-to-cell communication mechanism which regulates
virulence gene expression depending on cell population density. The global post-transcriptional regulator RsmA is essential for
this phenotype. Intracellular levels of the signal molecule c-di-GMP appear to modulate motility and attachment phenotypes, as
expressing c-di-GMP phosphodiesterases can compensate a loss of RsmA function. However, the molecular role of this signalling
molecule and its interaction with RsmA and quorum sensing system is not yet well understood. It is hypothesized that elevated
level of c-di-GMP inhibits swarming in
rsmA
mutants. The major goal of this study was to identify potential c-di-GMP receptors
mediating this control. This study identified a set of genes potentially coding for proteins acting as such c-di-GMP receptors by
using random transposon mutagenesis technique. Four genes disrupted by transposon insertion were found to restore swarming
in an
rsmA
mutant:
rhlC
, encoding for a rhamnosyltransferase involved in the production of rhamnolipd biosurfactants;
mexT
, a
transcriptional regulator controlling multi-antibiotic efflux systems,
cupA3
encoding a fimbrial biogenesis usher protein involved
in surface attachment and PA3866 encoding a bacteriocin. In addition, the investigation of the roles of a variety quorum sensing
signalling molecules on the restoration of swarming motility in a signal-less mutant was carried out. It was found that the fatty
acid chain lengths of N-acyl-homoserine lactones (AHLs) have important roles in controlling the swarming network. AHLs with
long fatty acid chain lengths were not found to act as biosurfactants during swarming.
sozy.baban@gmail.comDissecting the genetic basis of swarming motility
in
Pseudomonas aeruginosa
by transposon muta-
genesis technique
Soza Tharwat Baban
The University of Nottingham, United Kingdom
J Infec Dis Treat 2019, Volume: 5
DOI: 10.21767/2472-1093-C1-009