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Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
7
t h
E u r o s c i c o n C o n f e r e n c e o n
Clinical Pathology
and Epidemiology
F e b r u a r y 2 7 - 2 8 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
Clinical Pathology & Epidemiology 2019
O
ne of the most serious zoonotic disease for human and animals throughout the world Brucellosis has been listed by WHO
as one of the seven neglected zoonoses in the developing countries. The disease has been reported from almost all states
in India. Devoid of classical virulence markers, the
Brucella
genome inhabits several putative virulence genes identification of
which is important for better understanding of pathogenesis. These genes regulate their intracellular trafficking and survival to
establish a chronic infection protected from immune and antibiotic arsenals. Prolonged treatments with combination antibiotics
like doxycycline, rifampicin and streptomycin, which are active intracellular besides others, have been recommended to treat
cases of human brucellosis. In general, antibiotic resistance in
Brucella
is not observed though sporadic cases of antibiotic
resistance have been reported. The present study was undertaken to detect the putative virulence genes and
in vitro
antimicrobial
sensitivity pattern of
Brucella
isolates from India. Totally, 45 isolates of
Brucella
spp. (B.
abortus
16; B.
melitensis
29) from
human and animals from different regions of India namely Himachal Pradesh, Jammu & Kashmir, Punjab, Karnataka, Tamil Nadu,
Maharashtra, West Bengal, Assam and Meghalaya were used. Virulence gene profiling for nine genes, viz., VirB5, BtpA, BtpB,
VecC, BetB, BPE275, VirB2, BSPB and PrpA by polymerase chain reaction (PCR) and minimum inhibitory concentration (MIC)
of seven antibiotics, viz., rifampicin, azithromycin, tetracycline, ciprofloxacin, streptomycin, ofloxacin and co-trimoxazole using
the gradient diffusion susceptibility method (HiComb strips, HiMedia) was estimated. Virulence genes like VirB5 (37.77%), BtpA
(73.33%), BtpB (68.88%), VecC (55.55%), BetB (82.22%), BPE275 (77.77%), VirB2 (97.77%), BSPB (82.22%) and PrPA (71.11%)
could be identified among isolates. All the isolates were found sensitive to azithromycin, tetracycline, ciprofloxacin, streptomycin
and ofloxacin. Of these, 10 (B.
abortus
4; B.
melitensis
6) isolates exhibited intermediate sensitivity to co-trimoxazole. Resistance
to co-trimoxazole was found in 20 of which, 14 were B.
abortus
and 6 were B.
melitensis
. Similarly, 15 isolates were resistant
to rifampicin, which include B.
abortus
(10) and B.
melitensis
(5). Significantly, six isolates (B.
abortus
5; B.
melitensis
1) were
found resistant to both rifampicin and co-trimoxazole. While presence of combination of virulence genes may have influence on
the pathogenesis, resistance to antibiotic(s) of choice used to treat human cases needs attention. Implication of these would be
discussed.
sonidoimari@yahoo.co.inInvestigation into virulence genes and
antimicrobial sensitivity of field isolates of
Brucella from India
Soni Doimari, Vijayata Singh, Reena Kumari, M Suman Kumar
and D K Singh
ICAR-Indian Veterinary Research Institute, India
J Infec Dis Treat 2019, Volume: 5
DOI: 10.21767/2472-1093-C1-009