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Journal of Organic & Inorganic Chemistry
ISSN 2472-1123
2
n d
E d i t i o n o f E u r o S c i C o n C o n f e r e n c e o n
Chemistry
F e b r u a r y 1 9 - 2 0 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
Chemistry 2019
Introduction:
L1 cell adhesion molecule (L1CAM) expression has been reported to be associated with high-grade disease and
non-endometrioid histology, as well as poor prognosis, in endometrial carcinoma (EC). These high-risk EC types have already
spreaded outside the uterus when diagnosed and, after an extensive surgery, are often treated with chemo and radiation therapy.
We hypothesized that L1CAM gene expression could discriminate, among poor outcome EC patients, those who do and who do
not respond to adjuvant platinum-based chemotherapy.
Experimental Model:
Using an efficient multiplex qRT-PCR, we studied L1CAM mRNA expression on 117 EC and 16 normal
endometrial (NE) flash-frozen tissues, with HPRT1 and PPIA as reference genes.
Results:
L1CAM mRNA was significantly overexpressed in EC compared to NE tissues (p=0.02), significantly upregulated in G3
vs
. G1-2 ECs (p<0.001) and in non-endometrioid vs. endometrioid ECs (p< 0.001). Our analysis showed no difference in L1CAM
expression of stage I-II vs. stage III-IV ECs (p=0.5). Of the initial 117 EC patients, 47 received chemotherapy on adjuvant setting
and were classified as platinum-sensitive and platinum-resistant patients, based on PFI>12 months and <6 months, respectively.
L1CAM gene was significantly overexpressed in resistant vs. sensitive EC (p=0.001). Moreover, by means of a multivariate logistic
regression model, we found L1CAM gene overexpression as an independent indicator of the probability to harbor a platinum-
resistant EC (p=0.047, OR=3.5). In addition, univariate and multivariate survival analysis showed L1CAM gene upregulation is
associated with poor outcome, in terms of progression-free survival and disease-specific survival.
Conclusions:
Our results suggest L1CAM gene expression as a potential prognostic marker and a predictive biomarker of platinum
response in high-risk EC patients.
bignottieliana@gmail.comL1CAM gene overexpression is associated with
platinum resistance in high-risk endometrial
carcinoma
Bignotti E
1,2
, Ravaggi A
2,3
, Ratti M
1
, Tognon G
1
, Todeschini P
1,2
,
Zanotti L
2,3
, Sartori E
3
, Odicino F
3
and Romani C
4
1
ASST Spedali Civili di Brescia, Italy
2
IMM A Nocivelli-ASST Spedali Civili di Brescia, Italy
3
Division of Obstetrics and GynecologyUniversity of Brescia, Italy
4
University of Brescia, Italy
J Org Inorg Chem 2019, Volume: 5
DOI: 10.21767/2472-1123-C1-021