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Volume 10 Journal of Archives of Medicine Advanced Biotechnology & Annual Pediatrics 2018
November 28-29, 2018
Novel Trends and Advances in Biotechnology,
Cell & Stem Cell Research
15th Annual Congress on Pediatrics
World Congress on
&
November 28-29, 2018 Barcelona, Spain
Joint Event On
The role of TGF-β1 in the development of Fetal Alcohol Syndrome (FAS)
Zhanna Malakhova
Baltic Federal University, Russia
The poor results of all anti-alcohol programs create a chain of other problems. However, none of them is as burning as female
alcoholism and, consequently, the development of FAS in their children. The study aimed at identifying possible differences
in the TGF-β1 content in the blood serum of pregnant women who abuse alcohol and who do not. Twenty-nine pregnant
women were examined. Of these, 1st group (the main group) included 8 women (with a history of alcohol addiction), and 2nd
(control) group of 21. The determination of the TGF-β1 level was performed during 11th -14th weeks of pregnancy. Due to the
peculiarities of the examined women pregnancy period (gestosis, extragenital pathology and gynecological diseases), a parallel
study of TGF-β1 in rats was conducted. The investigations were carried out in the autumn-winter period on 26 1-2-month-
old Wistar rats, weighing 280-300g. They were divided into two groups: 1 - main (13 animals receiving 15% alcohol solution
instead of water for 1 month before pregnancy and during the entire pregnancy), 2 - control (13 intact rats). To determine
TGF-β1, a heterogeneous solid-phase enzyme-linked immunosorbent assay (Rat-TGF-β1 ELISA test system BMS623 and
BMS623TEN, Bender medsystems, Austria) was used. The number of TGF-β1 in the 1st group of women was 71.7 ng/ml, 8.8
ng / ml (t = 1.94, p <0.05) in the second. The reference values of TGF-β1 0 - 3.46 ng/ml. The experiment on rats also resulted in
statistically significant TGF-β1 value differences: in the experimental group M = 187.9 ng / ml, in the control group m = 129.7
ng / ml, t = 2.68, p <0.02. An increase in TGF-β1 concentration in women who took alcohol during pregnancy and a similar
trend in alcoholized pregnant rats allow to say that the existing receptor block to the growth factor is associated with ethanol
influence. Based on these provisions, it becomes possible to explain the complex of clinical data peculiar to newborns with FAS:
intrauterine growth retardation, facial deviations, changes in the central nervous system.
Biography
Zhanna Malakhova has completed his Graduation from the Pediatric Faculty of the Ural State Medical Institute in 1992. From 1996 he worked like Assistant of
the Department of Children's Diseases of the Medical Faculty of the Ural medical University. Since 2015 he has been working at the Baltic Federal University
(Kaliningrad) as a Professor of the Department of therapy.
Zhanna Malakhova, Arch Med 2018, Volume 10
DOI: 10.21767/1989-5216-C2-006