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Page 36
Volume 10
Journal of Archives of Medicine
Advanced Biotechnology & Annual Pediatrics 2018
November 28-29, 2018
Novel Trends and Advances in Biotechnology,
Cell & Stem Cell Research
15
th
Annual Congress on Pediatrics
World Congress on
&
November 28-29, 2018 Barcelona, Spain
Joint Event On
Bio-efficiency of
Cinnamomum verum
loaded niosomes and its microbicidal and mosquito larvicidal
activity against
Aedes aegypti, Anopheles stephensi
and
Culex quinquefasciatus
(Diptera:
Culicidae
)
Asaithambi Kalaiselvi
1
, Michael Gabriel Paulraj
2
, Savarimuthu Ignacimuthu
2
and Ekambaram Nakkeeran
1
1
Sri Venkateswara College of Engineering, India
2
Loyola College, India
T
he emergence of mosquito vector borne diseases is considered as a perpetual problem globally in tropical countries.
Outbreak of several diseases such as chikungunya, zika viral infection and dengue fever has created a massive threat
towards living population. Frequent usage of synthetic insecticides like DDT eventually had its adverse harmful effects on
humans as well on the environment. Since there are no perennial vaccines, prevention, treatment or drugs available for these
pathogenic vectors, WHO is more concerned in eradicating their breeding sites effectively without any side effects on humans
and environment by approaching plant derived natural eco-friendly bio-insecticides. The aim of this study is to investigate
the larvicidal potential
Cinnamomum verum
essential oil (CEO) loaded niosomes were prepared using trans-membrane pH
gradient method using cholesterol and surfactant variants of Span 20, 60 and 80 and treated against II to IV instar larvae of
Aedes aegypti, Anopheles stephensi
and
Culex quinquefasciatus
. The synthesized CEO loaded niosomes were characterized by
zeta potential, particle size, Fourier Transform Infrared Spectroscopy (FT-IR), GC-MS and SEM analysis to evaluate charge,
size, functional properties, composition of secondary metabolites and morphology. The Z-average size of the formed niosomes
was 1870.84 nm and had good stability with zeta potential -85.3 meV. The entrapment efficiency of the CEO loaded niosomes
was determined by UV-Visible Spectrophotometry. Evaluation of synthesized niosomes against gram-positive (
Bacillus subtilis
)
and gram-negative (
Escherichia coli
) bacteria and fungi (
Aspergillus fumigatus
and
Candida albicans
) at various concentrations
were assessed for its bio-potency. The larvicidal activity was evaluated at various concentrations for 24 h and LC50 and LC
90 values were calculated. The results exhibited that CEO loaded niosomes has greater efficiency against mosquito larvicidal
property and also the results suggest that niosomes could be used in various applications of biotechnology and drug delivery
systems with greater stability by altering the drug of interest.
dr.kalaiselvikarthik@gmail.comArch Med 2018, Volume 10
DOI: 10.21767/1989-5216-C2-006