The lack of Fam83h mediated reduction of Wnt/B-Catenin signaling pathway and expression levels of dental mineralization genes

Background: FAM83H has been identified as an essential gene for dental enamel formation and may be related to Wnt/β-catenin. Methods: levels of Fam20a, Dspp, Dmp1, Enam, Ambn, Sppl2a, Mmp20, Fgf10, and the mediators of Wnt/βcatenin pathway were measured in the dental root of both Fam83h-KnockOut and wild-type mice by using QPCR at 5,11 and 18 days after birth. The expression of Fgf10 and the mediators of Wnt/β-catenin were also evaluated in the skin of KnockOut and wild-type mice by using Q-PCR and also, The histology of hair follicles was compared. the Fam83h-KnockOut mice recruited in this study confirmed by Sanger sequencing and western blot analysis. Conclusion: it seems that in the lack of Fam83h, dental mineralization is induced by simultaneous decrease of Wnt / β-catenin mediators and the mineralization-related genes, suggesting to act in a cumulative effect manner and probably behave as a multi-factorial trait.

Author(s): Sherko Nasseri

Abstract | Full-Text | PDF

Share This Article