Abstract

Susceptibility of Clinical Bacterial Isolates and Control Strains to Nigella Sativa Oil

The clinical bacteria of multi-drug resistant to some antibiotics are considered a common problem in the world wide. Alternative antibacterial strategies are urgently needed, and thus this situation has led to a re-evaluation of the therapeutic use of ancient remedies, such as plants and plant-based products, including Nigella sativa (N. sativa). This study aimed to evaluate the antibacterial activity of Yemeni N. sativa oil against some Clinical Bacterial Isolates (CBI) and Control Strains of Bacteria (CSB) in comparison with Augmentin® (Amoxicillin and Clavulanic Acid). This study was carried out in Sana’a city during the period of one year from May 2012 to April 2013. The antibacterial activity of oil with different concentrations was determined by using disc agar diffusion technique against CBI (Staphylococcus aureus and Pseudomonas aeruginosa) and CSB (Staphylococcus aureus ATCC25619, Pseudomonas aeruginosa ATCC29737), also the Minimum Inhibition Concentration (MIC) of oil extract was determined. The results indicated that, the highest significant antibacterial activity was at the maximum concentration of the oil against Staphylococcus aureus and Pseudomonas aeruginosa in comparison with Augmentin ® discs (p < 0.05) respectively. This study observed that, there was strong positive correlation between the diameter zone (DZ-mm) and oil concentrations (%). In addition, the DZ (mm) of the oil for CSB was higher than CBI. Also the oil activity was found to be more effective against Staphylococcus aureus than Pseudomonas aeruginosa. On the other hand, the MIC of the oil against CBI were ranged between ≥ 0.5% and 4%. Whereas, the CSB particularly Staphylococcus aureus were sensitive to low concentrations of oil. It could be concluded that, the oil had high antibacterial effect against CSB and CBI with high concentration and more effective than Augmentin® discs.


Author(s): Majdi Al-Somat, Adnan Al-Adhal, Najeeb Ghanem, Khaled Al-Moyed, Abdullah Shobit and Mohammed Amood AL-Kamarany

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