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Study of the volatile compounds in Malabaila Isfahanica from Iran using HS/SPME/GC/MS

The aerial parts of the plant Malabaila Isfahanica were collected in July 2010 from Binaloud mountain (Khorasan Razavi Province of Iran ). It is air dried in a shadow place. A headspace solid-phase micro-extraction (HS-SPME) method followed by gas chromatography–mass spectrometry (GC/MS) was used for the analysis of volatile compounds in the leaves of Malabila Isfahanica. The dried plant was powdered and the aroma compounds of a 0.05g were isolated by HS-SPME fiber in 15 min. The chemical compounds of the plant were investigated by gas chromatography mass spectrometry (GC/MS). A total of 13 constituents, representing more than 94% of the volatiles were identified. The main compounds, were limonene oxide [(16.80%) , 3- buten-2-one,4-[2,6,6-trimethyl-1-cyclohexen-1-yl] (16.44%), β -cyclocitral (12.56%)and β- pinene (12.41%). This herbal medicine traditionally uses as anti-inflammatory in Iran. Consequently, this fast and simple method can be used for the analysis of the volatile compounds emitted from Malabila Isfahanica
Author(s): A. Nezhadali1,2  , A. Nezhadali Baghan2

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