Studies on Antioxidant Activity and Total Phenolic Content of Tinospora cordifolia (Miers.) Stem Using in Vitro Models

In the present study, we examined the anti-oxidant effects of Tinospora cordifolia stem. Dried and powdered stem of Tinospora cordifolia was extracted with ethanol and methanol. Total phenolic content of different solvent extracts were determined, to find the correlation between phenols and antioxidant activity. Ascorbic acid was used as standard. Antioxidant assay was carried out by using DPPH (1, 1-Diphenyl-2-picrylhydrazyl) radical scavenging activity. Ethanolic stem extract showed the highest free radical scavenging activity (56.35%). The antioxidant activity of methanolic extract was poor when compared to the ethanolic extract. Ethanolic stem extract had the highest phenol content of 66.28 ± 0.82 mg/g. These results suggest that, Phytocompounds were better extracted in ethanol and there is direct correlation between the total polyphenols extracted and its antioxidant activity.

Author(s): Neha Upadhyay, Showkat Ahmad Ganie, Rajneesh K Agnihotri, Rajendra Sharma

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