Spectroscopic determination of hesperidine in ayurvedic formulation madiphal Rasayana

A simple and sensitive spectrophotometric method was developed for the determination of Hesperidine in API and ayurvedic formulation Madiphal Rasayana. Hesperidine with λmax 284.5nm obeyed Beer's law in the concentration range of 10-100μg/ml. An Assay method was developed for Madiphal rasayana and the percentage purity was found to be was 98.94% w/v. The proposed method was successfully validated as per the ICH guidelines for API and ayurvedic formulation containing Hesperidine. The percentage recovery studies were performed and the percentage recovery was found to be 98.27 - 99.70% w/v, the system was found to be linear and the correlation coefficient (r2) was found to be 0.999, method was found to be precise and the relative standard deviation was found to be 0.197, detection limit was found to 0.03μg/ml and quantification limit was found to be 1μg/ml respectively. The stress degradation studies were performed for the API as per the ICH guidelines, the degradation was observed in hydrolytic degradation under acidic and alkaline conditions, dry heat induced degradation, oxidative and photolytic degradation.

Author(s): Sandeep Kumar Naik B., Duganath N., Rubesh Kumar S., Bharath Ratna Kumar P. and Devanna N.

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