Simultaneous spectrophotometric estimation of aceclofenac and thiocolchicoside by first order derivative method in combined dosage form

The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. first order derivative method for the determination of aceclofenac and thiocolchicoside in combined dosage form i.e. tablets by using methanol as a solvent. The method was further validated by ICH guidelines. The proposed first order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 259 nm and 353.6 nm were selected for the estimation of aceclofenac and thiocolchicoside respectively. The linearity of the proposed method was found in the concentration range of 1 to 14 µg /ml (r2= 0.9995) for aceclofenac and 1 to 14 µg /ml (r2= 0.9999) for thiocolchicoside respectively. The percentage mean recovery was found to be 100.52 % for aceclofenac and 100.72% for thiocolchicoside and respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method.

Author(s): Rajan V. Rele

Abstract | PDF

Share This Article
30+ Million Readerbase
Google Scholar citation report
Citations : 374

Der Pharmacia Sinica received 374 citations as per Google Scholar report

Abstracted/Indexed in
  • Google Scholar
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • Directory of Research Journal Indexing (DRJI)
  • Proquest Summons
  • International Committee of Medical Journal Editors (ICMJE)
  • Serials Union Catalogue (SUNCAT)
  • Geneva Foundation for Medical Education and Research
  • Secret Search Engine Labs

View More »

Flyer image