Previous epidemiologic studies suggested that vitamin D deficiency may be a risk factor of obesity and type 2 diabetes mellitus (DM). However, the result is still controversial. Type 2 DM and obesity are widely prevalent in Ukraine. The aim of the study was to determine the vitamin D status in relation to type 2 DM and obesity. This cross-sectional study was conducted at the Bukovinian State Medical University from June to September 2019. Approval of ethical committee was taken. A total of 402 patients with type 2 DM and obesity were enrolled in this study. Vitamin D status was assessed using 25(OH)D levels. Height and weight of the patients were recorded to obtain BMI. Of the total sample, 283 (70.4%) were females and 119 (29.6%) were males. Mean age of the participants was 47.28±14.83 years. Mean vitamin D levels were 19.03±6.91 ng/ml. A total of 373 (92.8%) patients were either vitamin D deficient 171 (42.5%) or insufficient 202 (50.2%) while 29 (7.2%) were sufficient in vitamin D. As regards BMI and vitamin D status, the difference was statistically significant (p=0.014). Vitamin D deficiency is widely prevalent in Ukraine. There is also a high prevalence of obesity and type 2 DM and they are inversely related to low vitamin D levels.