Preliminary Bacteriological Evaluation of Abattoir Waste water Treated With Moringa Oleifera Seed Powder

Objective: The used of synthetic chemicals in water treatment is expensive and raises fears on health implications and environmental safety, the need to find alternatives that are safe health-wise and environmentally friendly gave rise to this study.
Methods: A preliminary bacteriological evaluation of abattoir waste water treated with powder obtained from M. oleifera was carried out. Isolation and biochemical techniques were employed to identify pathogenic organisms. The M. oleifera seeds were dried in the oven at 40°C to constant weight. They were winnowed, shelled and grounded using sterile mortar and pestle. A sufficient powderwas obtained using a 400mm size sieve. Before treatment a 1ml aliquot of the waste water sample was serially diluted, the 10⁷ and 10⁸ were cultured on Nutrient agar, MacConkey agar and Eosin Methylene Blue agar and the plates were incubated at 37±2⁰C for 24 hours. Plates containing colonies ranging from 30 – 150 were counted and recorded at 24 hours interval for 3 days to obtain the Total Bacterial Count (TBC), Total Coliform Count (TCC), Escherichia coli Count (ECC) before and after treatment.
Results: Different concentrations of the powder (1.0g, 2.5g, and 5.0g) were used to treat 100ml of the waste water resulting in a gradual change in colour with increasing concentration and time. TBC, TCC, ECC fallen to ≈ 4.4 x 10⁷, 3.2 x 10⁵and 1.6 x 10⁴ respectively. After 48 hours, TBC, TCC, and ECC fallen to ≈ 2.2 x 10⁷, 3.1 x 10⁵ and 1.1 x 10⁴ respectively. After 72 hours TBC, TCC and ECC fallen to 2.1 x 10⁷, 1.7 x 10⁵ and 1.5 x 10⁴ respectively. On treatment with 2.5g, after 24 hours the TBC, TCC and ECC reduced to 2.3 x 10⁷, 3.5x 10⁵ and 1.1 x 10⁴ respectively. After 48 hours TBC, TCC and ECC fallen to 2.1 x 10⁷, 2.5 x 10⁵ and 0.5 x 10⁴ respectively. After 72 hours TBC, TCC, ECC fallen to 2.0 x 10⁷, 1.5 x 10⁵, and 0.3 x 10⁴ respectively. After 24 hours of treatment with 5.0g, TBC, TCC and ECC fallen to 2.0 x 10⁷, 2.9 x 10⁵ and 0.9 x 10⁴ respectively. After 48 hours, TBC, TCC and ECC fallen to 1.1 x 10⁷, 2.4 x 10⁵ and 0.2 x 10⁴ respectively. At 72 hours TBC, TCC and ECC fallen to ≈ 1.0 x 10⁷, 1.1 x 10⁵ and 0.1 x 10⁴ respectively.
Conclusion: This investigation shows that the powder obtained from M. oleifera seed can also be used effectively in the management of abattoir waste water.

Author(s): ASEMOTA, U.K, ODUTAYO, O.I, OYEWOLE, I.O AND ABUBAKAR S.

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