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Phytochemical Analysis and In vitro Antioxidant Properties of Murraya koenigii (L.) Fruits

Objective: The present study was undertaken to evaluate the phytochemical and In vitro antioxidant property of fruits extract of Murraya koenigii (FEMK). The present study also evaluated the presence and quantification of flavonoids contents in crude extract.
Methods: The in vitro antioxidant activity was performed using inhibition of superoxide anions; free radicals scavenging activity by DPPH, H2O2, NO, reducing power assay and lipid peroxidation method. The investigation was also carried out to evaluate the presence and quantification of flavonoids contents in crude extracts. HPTLC fingerprint profile of FEMK was developed which serves as reference standard for quality control of the extracts.
Results: The results of antioxidant activity of Fruit extracts of Murraya koenigii (FEMK) demonstrate to scavenge the free radicals in all in-vitro models indicates antioxidant property. The observed effects of extract could be due to the presence of flavonoids. The flavonoids content was found to be 0.117 %, 0.206 %, and 0.348% w/w in 5mg/ml, 10 mg/ml and 15 mg/ml concentrations of FEMK respectively. Conclusion: The study concludes that the Fruit extract of Murraya koenigii shows moderate to strong antioxidant activities. Thus fruit extract of Murraya koenigii could be useful for the application of disorders related to free radicals.

Author(s): Atish N. Waghmare, Sachin V. Tembhurne and Dinesh M.

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