Isolation of multidrug-resistant Staphylococcus aureus strains from various body sites in patients with different infections

Staphylococcus aureus is one of the most prevalent causative agents of infections and linked to increasing mortality associated with multidrug resistance worldwide. This bacterium is an important cause of endovascular infections, pneumonia, osteomyelitis, septic arthritis, skin and soft tissue infections, endocarditis, foreign-body infections and sepsis. S. aureus is a common cause of infections in patients in intensive care units in many countries. One of the reasons for the success of this human pathogen is its variability, occurring at different times and countries. The aim of study was to identify frequency of multidrug-resistance S. aureus (MDRSA) strains isolated from different sites of patients with various infections, to determine prevalent body sites. The average age of patients was 40 years. Strains were examined according to morphological, microscopical, tinctorial parameters. Cultivation of S. aureus were performed on different media (meat-peptone agar, meat-peptone broth, 5% blood agar, egg yolk-salt agar, milksalt agar); cultivation ability was also studied according to temperature, biochemical properties (carotenoid pigment formation, carbohydrate fermentation, enzyme activity) and antibiotic susceptibility tests. Study results showed that the most prevalent site was oral cavity-MDRSA cultured in 19.35±2.98% cases. By comparison, isolation was not significantly different for pharyngeal and soft tissue infections (the same number for both sites-14.44±2.93%). There were detected a few and equal number of isolation cases from peritoneal liquid and phlegmon samples (both- 2.15±1.42%). In other cases number of isolated strains varied between above mentioned data (skin and soft tissue infection-6.09±2.25%, nasal cavity-5.73±2.10%, eye- 4.65±2.01%, ear-3.94±1.87%, trachea-3.94±1.87%, urine-3.58±1.79%).

Author(s): Shorena Khetsuriani

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