Isolation of chlorogenic acid from the stems of Viburnum coriaceum Blume

The genus Viburnum Linn. belonging to the family Caprifoliaceae, consists of about 20 species throughout the world. Many of these species have been reported to possess phenolic compounds and their glycosides as their major chemical constituents in their stem and root barks, which may be attributed to their remarkable biological activities against smooth muscle related troubles. The main objective of the present study is to detect, isolate and characterize some phenolic compounds from aqueous ethanolic stem extract of two Viburnum Linn. Species collected from Nilgiri hills, Tamilnadu, India. The dried stems of Viburnum coriaceum Blume. were powdered and extracted separately and successively with petroleum ether (60 – 80°C), chloroform and 75% aqueous ethanol. The ethanolic extracts were screened by Thin Layer Chromatographic techniques for the presence of different classes of phenolic compounds. The TLC studies revealed the presence of some flavonoid which was later confirmed to be chlorogenic acid in V.coriaceum by co-TLC technique against the reference substances. The ethanolic residues were adsorbed on the activated neutral alumina and packed in a glass column containing slurry of alumina in acetone. Then, the column was eluted firstly with some non-polar and moderately polar solvents to clear out impurities which remain present in the column followed by a gradient elution with methanol-water mixture. The fractions yielded 35 mg of chlorogenic acid from V.coriaceum. The samples were subjected IR, NMR spectral analysis to evident their molecular structures.

Author(s): K Prabhu, P K Karar, S Hemalatha, K Ponnudurai

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