Objective: To investigate the influence of selected metal ions CaCl2, BaCl2, SrCl2, ZnCl2, CoCl2, CuCl2 and FeCl3 on the effect of acetylcholine on nicotinic receptors of skeletal muscle of the frog. Since nicotine also acts on nicotinic receptors, the influence of metal ions on nicotine was also studied.
Method: The dose-response curve of acetylcholine and nicotine was obtained by using isolated rectus abdominis muscle of frog. The influence of various concentrations of selected metal salt solutions (1-1600μg) was tested on selected submaximal response of acetylcholine and nicotine.
Results: CaCl2 potentiated the effects of both acetylcholine and nicotine. But BaCl2 and SrCl2 improved acetylcholine activity without altering nicotine response whereas CoCl2 and ZnCl2 reduced nicotine action without modifying acetylcholine action. The ions of CuCl2 and FeCl3 did not alter the contraction produced by both acetylcholine and nicotine.
Conclusion: The study demonstrates that supplementation of CaCl2, BaCl2 and SrCl2 may be useful in the case of myasthenia gravis and metal ions can be used to distinguish the acetylcholine action from that of nicotine at NMR.