Abstract

Free Radical Scavenging Activity and Histochemical Localisation of Reactive Oxygen Species in Fenugreek Sprouts Primed with Nitric Oxide Donors

Objective: Nitric oxide (NO) is considered as an important signal molecule in plant system. In the present study, the effect of some elicitors namely, sodium nitroprusside (SNP), sodium nitrite (NN) and potassium ferricyanide (FCN) on reactive oxygen species localisation and the antioxidant activity of Trigonella foenumgraecum L. was studied during germination. Method: The antioxidant activity and reactive oxygen species localisation were evaluated up to 3 days at an interval of 24h. The in vitro antioxidant property was determined by the following spectrophotometric methods: scavenging of DPPH and ABTS+ radicals, ferric reducing power and metal chelating property; also phytochemicals like phenol and flavonol contents were determined.
Results: The results demonstrated that the donors of nitric oxide treated seeds showed increase in their antioxidant activity from 24h to 48h and then after declined at stage of 3 days. Overall, the seeds treated with potassium ferricyanide and sodium nitroprusside exhibited higher antioxidant property when compared with that of control. It was evident from the study of histochemical localization of H2O2, lipid peroxidation and plasma membrane integrity that sodium nitroprusside and potassium ferricyanide exhibited protective role in the germinating seedlings of fenugreek. Conclusion: Our result supported that nitric oxide plays an important role to elicit antioxidant components in plants.


Author(s): Saran Kumar Gupta and Palash Mandal

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