The antimicrobial activity of different solvent extracts (hydro alcoholic extracts of methanol) of different parts of Lagenaria siceraria (Molina) Standl was evaluated for its antimicrobial potential. The in vitro antimicrobial activity was evaluated by agar well diffusion method. Extraction was done by individual cold percolation method. The antimicrobial activity was tested against five Gram positive bacteria, five Gram negative bacteria and four fungi. The MIC and MBC was also determined, including five antibiotics. All the parts possessed antimicrobial activity but to a different level. The extracts showed better antifungal activity than antibacterial activity and Gram negative bacteria were more susceptible than Gram positive bacteria. The peel showed lowest MIC and MBC values indicating the therapeutic value of agro waste material.