Most of the traditional systems of medicine are effective but they lack of standardization. So, there is a need to develop a standardization technique. Central Council of Research in Ayurveda and Siddha has given preliminary guidelines for standardizing these conventional formulations. For the uniformity in production of herbal formulations it is necessary to develop methods for evaluation. In the paper, attempt had been made to evaluate Dhouti-Yog Churna, an Ayurveda formulation. One sample was procured from manufacturer and was subjected to compare with in house preparation sample by performing physicochemical screening, phytochemical screening, and microscopic characterization. It was observed that market samples matched with that of authentic standards. The inhouse formulation also produced the similar results with that of marketed formulation1.