Effect of a Benign Prostatic Hyperplasia (BPH) Xenobiotic - Croton Membranaceus M.Arg. Root Extract on CYP1A2, CYP3A4, CYP2D6, and GSTM1Drug Metabolizing Enzymes in Rat Model

Fifteen (15) castrated -testosterone BPH induced male Albino Wister rats weighing between 120 -150 grams were randomly divided into 3 groups of 5 rats each (Groups II-IV). Five (5) uncastrated rats Group I was used as a negative control, receiving only distilled water. Group II was administered 30 mg/kg b. wt extracts of C. membranaceus. Group III was the model group while Group IV was administered 0.5 mg/kg b. wt finasteride and it was used as the positive control. BPH was induced in groups II-IV by administering testosterone propionate, 3 mg/kg b. wt for 28 days after a 7-day post castration rest period. Rats were euthanized and individual livers harvested for microsome preparation. The liver microsomes were assayed for, Phase I Drug Metabolizing Enzymes (DME); CYP1A2, CYP3A4, CYP2D6, and phase II; GST-M1 using Enzyme-Linked Immuno-Sorbent Assay (ELISA) techniques.

Author(s): Ongong'A Royronald Ochieng*,George A Asare, Yvonne Anang, Richard H Asmah, Habibur

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