Discovery and Heterologous Expression of the Large GC-Rich Marinomycin Biosynthetic Gene Cluster

Marinomycins are polyene antibiotic isolated from marine microbe (Marinispora CNQ-140) with high activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VREF) known to be the causative organisms for most infectious diseases. In dim light Marinomycin A isomerizes to Marinomycin B and Marinomycin C with lesser potency against MRSA and VREF. Understanding the Marinomycins biogenetic gene cluster (BGC) has been a difficult task due to their large sizes and high GC content which makes the cluster susceptible to re-arrangement. Transformation of Streptomyces lividans by construct containing the cluster and heterologously expressing the encoded biogenetic cluster aided the production of Marinomycins.

Author(s): Matthias O. Agbo

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