Abstract

Development and Validation of a HPTLC method for determination of Karanjin in Pongamia pinnata: A novel Indian medicinal plant

Pongamia pinnata is a popular Indian medicinal plant used for treatment of bronchitis, whooping cough, rheumatic arthritis, and diabetes diseases. Karanjin in one of the prevalent phytoconstituent present in this plant. A sensitive, selective and precise thin-layer chromatographic method has been developed and validated for the analysis of Karanjin in Pongamia pinnata. Separation and quantification was achieved by TLC using mobile phase of ethanolethyl acetatate (7:3, v/v), (RF 0.69) on precoated silica gel 60F254 aluminium plates and densitometric determination was carried out in reflection/absorption mode at 260 nm. The calibration curve was linear in the concentration range of 0.1μg-1.2μg spot-1. The method was validated for precision, repeatability and accuracy. The proposed method was found to be simple, precise, specific, sensitive and accurate for the quantification of Karanjin.


Author(s): Sweety Lanjhiyana, Kartik C.Patra, Dheeraj Ahirwar, Avtar C. Rana, Debapriya Garabadu and Sanjay K. Lanjhiyana

Abstract | PDF

Share This Article
30+ Million Readerbase
Google scholar citation report
Citations : 374

Der Pharmacia Sinica received 374 citations as per google scholar report

Abstracted/Indexed in
  • Google Scholar
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • Directory of Research Journal Indexing (DRJI)
  • Proquest Summons
  • International Committee of Medical Journal Editors (ICMJE)
  • Serials Union Catalogue (SUNCAT)
  • Geneva Foundation for Medical Education and Research
  • Secret Search Engine Labs

View More »

Flyer image