Pongamia pinnata is a popular Indian medicinal plant used for treatment of bronchitis, whooping cough, rheumatic arthritis, and diabetes diseases. Karanjin in one of the prevalent phytoconstituent present in this plant. A sensitive, selective and precise thin-layer chromatographic method has been developed and validated for the analysis of Karanjin in Pongamia pinnata. Separation and quantification was achieved by TLC using mobile phase of ethanolethyl acetatate (7:3, v/v), (RF 0.69) on precoated silica gel 60F254 aluminium plates and densitometric determination was carried out in reflection/absorption mode at 260 nm. The calibration curve was linear in the concentration range of 0.1μg-1.2μg spot-1. The method was validated for precision, repeatability and accuracy. The proposed method was found to be simple, precise, specific, sensitive and accurate for the quantification of Karanjin.