Background: Autism is usually accompanied by many symptoms such as abdominal pain, constipation/diarrhea, due to deregulation of physiological microflora. Recently, regression of the autistic symptoms after oral administration of antibiotic such as vancomycin was detected, that leads to the disappearance of the symptoms and eliminate toxins produced by bacteria such as Clostridium spp.
Methods: In the present study we are identified the chemical compositions of non-polar fractions (PGp and PGc) from natural bee pollen by using GC/MS against AD induced by Clostridium perfringens in vitro.
Results: 23 and 14 chemical components representing 84.07% and 92.92% for PGp and PGc respectively by GC/MS. Total phenolic and proanthocyanidine contents were determined in both PGc and PGe fractions by Folin -Ciocalteau and vanillin-sulfuric acid assays respectively and expressed in term of quercetin equivalent (y=1.6463x + 132.6, R2=0.9972) as mg QUE/g fraction or gallic acid equivalent (y=0.0019x + 0.137, R2=0.9988) as mg GAE/g fraction and as (+) - catechin equivalent (% CT w/w) in bee pollen (y=0.0013x + 0.3998, R2=0.9997).
Conclusion: The results of the present study revealed the capacity of natural bee pollen to enhance the recovery from AD induced by C. perfringens in vitro. This is due to the diversity of different chain of fatty acids as well as phenolic/ proanthocyanidin compounds that found in bee pollen fractions.