Abstract

Chemical Composition and Antioxidant Potential of Polyphenol Compounds of Cyperus rotundus L. Rhizomes

The aim of the study is extracted and purity flavonoids from Cyperus rotundus rhizomes. Ethanolic extract obtained by removing the oily material from it by using soxhlet methods with of petroleum ether solvent and then wash with ethanol solvent in concentration 70%, the extract which confirms the presence of polyphenol and flavonoids. Purified flavonoids was obtained by gel filtration column (Sephadex LH-20).The ethanolic extract and purified flavonoids were examined by spectral diagnostic using Thin Layer Chromatography (TLC) and high performance liquid chromatography (HPLC) which showed the presence of flavonoids compound. The ethanolic extract and flavonoids were first subjected to phytochemical analysis followed by evaluation of their antioxidant potential by measuring the total phenolic content. The free radical scavenging activity of Rhizomes from Cyperus rotundus evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay the results revealed that activities of (BHT) synthetic antioxidant and vitamin C as free radical scavenging increased when is compared with flavonoid purified and ethanolic extract rhizomes, the free radical scavenging activity of flavonoid purified is less than synthetic antioxidant and flavonoid purified is highest free radical scavenging to ethanolic extract C. rotundus. Total phenolic of Cyperus rotundus sample ethanolic extract 10 and 25 mg/ml were 1.1758 and 2.0969mg/ml, respectively, and pure flavonoid 10mg/ml and 25mg/ml were 1.0159 and 1.1861mg/ml respectively. These results established the antioxidative potency of C. rotundus Rhizomes, which may account for some of the medical claims attributed to this plant.


Author(s): Essam F.A. Al-Jumaily and Jameelah K.T. Al-Isawi

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