Cancer Science & Pediatrics 2019: Insight into Sarcoma biology from Sarcoma Cell Line Progression Series- Jiri Hatina, Charles University Medical Faculty in Pilsen, Czech Republic

Soft tissue sarcomas are known for his or her great variability in clinical behaviour, starting from almost indolent lesions to rapidly metastasing tumours. Genes liable for  sarcoma progression are poorly characterized by now. Towards this end, we comprehensively analyzed transcriptomes of two single-background progression series of murine sarcoma cell lines. the primary one, established by us from consecutive fibrosarcomas during a v-jun transgenic mouse, consisted of the slowly proliferating nonmotile and noninvasive cell line JUN-2, rapidly proliferating, motile and invasive cell line JUN-3, and therefore the cell line JUN-2fos-3 that exhibits a singular transformation pattern, with little deregulation of cell growth and proliferation, but pronounced motility and invasiveness. The other, established by a french group, consisted of the widely used pre-adipocytic cell line 3T3L1 and its derivative liposarcoma cell line LM3D. From both liposarcoma cell lines, we isolated putative liposarcoma stem cells (as side-population cells). We performed four sets of genome-wide transcriptomic analyses. The JUN-fibrosarcoma progression series, by virtue of its unique distribution of transformation related-traits between individual cell lines, made us possible to spot two separate groups of genes tentatively involved in sarcoma progression during a single transcriptomic analysis on the one hand, proliferation-related genes might be identified by their differential expression in JUN-3 compared to both JUN-2 and JUN-2fos3, and, on the opposite hand, motility and invasiveness-related genes might be identified by their common expression pattern in JUN-2fos3 and JUN-3 cells compared to JUN-2.

Author(s): Jiri Hatina

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