Application of High performance thin layer chromatography-densitometry for the simultaneous determination of Metformin hydrochloride and Glipizide in bulk drug and tablet formulation

Method describes an HPTLC method for the simultaneous determination of glipizide and metformin HCl from tablet dosage form. This employes a precoated silica gel 60 F254 (0.2 mm thickness) on aluminium sheets and a mobile phase Ammonium sulfate (0.5%): water: methanol: ethyl acetate in the ratio of 5.0: 5.0: 2.5: 2.5 (v/v/v/v), having chamber saturation for 30 min at room temperature. The mobile phase was run upto 8cm. The Rf values were found to be 0.31 and 0.70 for glipizide and metformin HCl respectively. The plate was scanned and quantified at 245 nm. The linear detector response was observed between 100 ng.spot-1 to 500 ng.spot-1 and 1000 ng.spot-1 to 5000 ng.spot-1 for glipizide and metformin HCl respectively. The method so developed was validated for its accuracy and precision. The LOD and LOQ were found to be 24.5 ng.spot-1 and 80 ng.spot-1 for glipizide and 60 ng.spot-1 and 200 ng.spot-1 for metformin HCl respectively. The recovery was carried out by standard addition method. The Average recovery was found to be 99.96 % and 99.77% for glipizide and metformin HCl respectively.

Author(s): Shweta S. Havel and Sunil R. Dhaneshwar

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