Andrographis paniculata has obtained a well renowned name as one of the best traditional remedies, owing to its multiple pharmacological activities. In this work, the anti- histamine effect of hydroalcoholic leaf extract of Andrographis paniculata (Burm.f), family Acanthaceae, was planned. The concentration response curve (CRC) of histamine was recorded and its modification by anti histamine drugs of pheniramine maleate and hydroalcoholic extract of Andrographis paniculata Leaf (1mg/ml) by using guinea pig ileum preparation. The histamine dose responses were reduced (*P<0.005) after the incubation of hydroalcoholic extract of Andrographis paniculata Leaf (1mg/ml) when compared with normal CRC of histamine. The CRC of histamine was reduced (*P<0.05) after the incubation of pheniramine maleate (100μg/ml) when compared with normal CRC of histamine. The pheniramine maleate has been highlighted more anti histamine activity when compared with Andrographis paniculata leaf extract (APLE). Hence our present study was aimed to justify that the traditional claim of the entire leaf has used against histamine induced disease condition.