Analysis of chemical composition of pomegranate (Punica granatum) extracts by HPLC-DAD-ELSD and GC-MS

The pomegranate is known for its traditional use and medicinal since ancient times. It has health benefits for its various organs. Many studies have been done on its fruits, leaves and bark. Among the good properties, we mention the antioxidant1 and antimicrobial2 activities well known for fruit peel.

This study was devoted to the chemical characterization of pomegranate fruit bark extracts. Firstly, the dry material was ground and extracted successively with 4 solvents of increasing polarity (cyclohexane, dichloromethane, ethyl acetate, methanol). The extraction yield is low for the first three solvents (0.2-0.7%) and high for methanol (43%).

These different extracts were subsequently analyzed by HPLC-DAD-ELSD to characterize the phenolic and majority compounds in these extracts. A hundred phenolic standards were compared to the peaks of the different extracts.

In parallel, a characterization of the volatile chemical compounds was carried out by GC-MS by direct introduction and after derivation of the extracts by sillylation.

Author(s): Aina RAKOTONDRATSOAVINA1, Pierre Luc Puyjalon1, Hicham FERHOUT2, Jalloul BOUAJILA1

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