A simple and sensitive RP-HPLC method for simultaneous estimation of dexketoprofen and thiocolchicoside in combined tablet dosage form

This paper describes a simple, sensitive, accurate, and validated reverse-phase high-performance liquid chromatographic (RP-HPLC) method for the simultaneous quantification of these compounds as the bulk drug and in tablet dosage forms. Separation was carried out on Jasco HPLC system equipped with HiQ sil C₁₈ HS column (250 × 4.6 mm i.d.) and PDA detector using Methanol: Sodium acetate buffer (70:30, v/v) with pH adjusted to 5 with Glacial acetic acid as the mobile phase and detection was carried out at 265 nm. Results were linear in the range of 5-30 μg mL⁻¹ for Dexketoprofen and 1 –10 μg mL⁻¹ for Thiocolchicoside. The method was successfully applied for the analysis of drugs in pharmaceutical formulation. Results of the analysis were validated statistically and by recovery studies.

Author(s): Vandana S. Bhavnani, Santosh V. Gandhi, Padmanabh B. Deshpande, Abhijit D. Dhiware and Sachin E. Potawale

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