Abstract

A Comparative Study of Wheat Leaf Infection by Bipolaris Sorokiniana on Resistant and Susceptible Wheat Varieties using Scanning Electron Microscopy

Bipolaris sorokiniana is a phytopathogenic fungus that causes diseases in cerealcrops worldwide. In this study, the speed of infection by the fungus on wheat leafsurface tissues was observed using scanning electron microscopy. The fungus was isolated from diseased wheat plants and identified to be B. sorokiniana based onmorphological characteristics. Young leaves of wheat plants were point inoculatedwith a conidial suspension of B. sorokiniana, and infection process of the pathogenwas observed. The fungus germinated on wheat leaf surface within eight hrs ofinoculation and its germ tube was elongated in 12 hrs of inoculation. On theleaf surface, a hyphal mat was initiated within 24 hrs after inoculation with thinmycelial mat produced by 48 hrs. Moreover, germ tubes elongated more on thesurface of the resistant varieties. The infection rate and pathogenesis depended onhost resistance to the disease. This study provides scanning microscopic evidenceshowing that the fungal pathogen can grow speedily on wheat leaf surface within a short period of time, indicating its virulence on susceptible wheat varieties.


Author(s): Md Muzahid Rahman, Krishna Kanta Roy, K Mustarin , Md Sahin Ali, M.A. Reza, Pingsheng Ji and Emran Ali

Abstract | Full-Text | PDF

Share This Article