Starch is the most commonly used as excipient in pharmaceutical preparations. They are used as Diluents, fillers and disintegrant in tablets. The bioavailability of any drug in the formulation is affected by various excipient presents in it. In case of tablets formulation, binders plays very important role in dissolution as well as bioavailability of drug. In the present work a comparative study of the binding properties of naturally isolated pear and guava starch was done in tablet formulated by using famotidine drug. Starch was isolated from natural source and then it was used in 2% w/v, 4% w/v, 6% w/v and 8% w/v in formulations, as binding agent. Then formulated tablets were further evaluated for various parameters. The hardness and disintegration time of the formulated tablets was found to be increased with increase in starch concentration. Hardness of optimized formulation, in case of pear and guava starch was 6.5±0.11kg and 6.0±0.09 kg, disintegration time was 10.0±0.28 and 8.0±0.60 min and friability was 0.48±0.15 and 0.76±0.41 respectively. The cumulative drug release after one hour for tablets containing 4% w/v of pear and guava starch (optimized formulation) showed maximum drug release was about 80.69% and 83.54±0.15 respectively. Then optimized formulation used for in vivo study. From the results, it was observed that, both pear and guava starch has significant binding characteristics. The percentage cumulative release of drug shows that guava starch has better binding properties then pear starch and both isolated starch was used as binding agents in pharmaceutical formulations for future aspects.